Aseptic Technique and Culturing Microbes 1

Aseptic Technique and Culturing Microbes 1



hi this is Janelle Martinez it's July 9th 2019 and I am working on the aseptic technique and culturing microbes lab so I have everything set here and I'm ready to start the experiment before my candle goes out so what I'm gonna do first I already have my sterile pipette in my hand I'm gonna take the e.coli the the broth – and just sterilize the the lip so we'll do that okay and then I'm just gonna set that down while I sterilize my culture vial so I'm gonna sterilize that and get it ready to put my broth in and then what I'm gonna do after that is measure out 0.25 milliliters of the broth and sterile ii put it into my culture while just making sure not to touch the edges here so there is 0.25 I'm going to set that down and drop that in and the tricky right that's this is sterile so and then I'm going to sterilize it one last time prior to closing it just to make sure it's ready to be shut and then I'm gonna shake it until it's completely dissolved after that it's gonna go right back into the broth tube so it's not completely dissolved I think I lost a few drops there so I'm going to add a little more there we go perfect no it's falling apart there just had to add a couple more drops there to get the results I wanted so now that's completely dissolve just want to be sure okay so I'm gonna sterilize that one last time for the transfer now my broth top is still sterile so let me get all of this solution here and I'm gonna drop that into the broth perfect now this can go into my alcohol make sure it gets in there and get it ready for the next step and then before I close this I'm gonna sterilize it one last time okay now it's ready for me too shut and I give it a little swirl there so I'm gonna put this aside that's done I'm gonna repeat the process now with my s epidermidis and then after that I'm gonna add the yeast which takes about 10 minutes and then follow the process from there okay and then I will have another video with my results thank you

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